Gelase instead of Geneclean

[BENNY SHOMER 9238]

Mary Metzler, Plants, x8076 (METZLER at ALA.BTK.UTU.FI) wrote:
: Has anyone tried Gelase, which is an enzyme which digests LM agarose,
: which eliminates the necessity of using Geneclean? I am tired of the
: low yields and unreliability of Geneclean. I also sometimes have to
: work with big fragments which aren't compatable with Geneclean. Has
: anyone used Gelase? You are supposed to be able to simply ethanol
: precipitate the DNA in your gel slice after digesting with this
: enzyme for an hour. Does anyone know if it works, and if it is
: expensive?
: Thanks,
: 	Mary Metzler
: 	University of Turku
: 	Turku, Finland
: 	Metzler at sara.cc.utu.fi

 Recently, I have stopped using all these methods. I use a modification of 
a method that was posted here recently . I cut and load about 20% more then
I need. Run the gel, cut out the band of interest with a minimum agarose.
Put the slice in a PCR tube which contains some glass fibers,
and freeze (liquid N , or Dry ice). Make a hole
with 25g needle (I make it bottom AND lid). Put tube into a 1.5ml tube and
spin (I do 15min' at least). The DNA is at the 15ml tube nice and clear.

Since it is very easy, and yields are good and reproducible, I now use it
regularly.

BTW, the original method posted, also used a special buffer to be added with
the band. I ommit this one. I don't know if that affects the yield. 

Hope this helps. Benny.
 **************************************************************
* Benny Shomer                                                 *
* Tel-Aviv University                                          *
* Sackler School of Medicine, Dept.of Embryology and Teratology*
*--------------------------------------------------------------*
* Snail:  Ramat-Aviv , Tel-Aviv  69978 ,  Israel.              *
* E-mail :  pc386 at ccsg.tau.ac.il                               *
* Tel :  972-3-640-9238     FAX :   972-3-642-2046             *
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