Long Ranger seq. gel solution.

Jim Wellehan James.F.X.Wellehan at dartmouth.edu
Fri Jul 16 17:55:44 EST 1993


In article <1993Jul15.101017.7179 at gserv1.dl.ac.uk>
daj at nhm.ic.ac.uk ((David Johnston) daj) writes:

> We also fix/wash our gels in 3L of 10% methanol 10% glacial acetic (despite 
> the standard protocol saying you don't need too - we have never had a gel 
> we could afford to loose by trying it out).

You can trust the protocol on that.  It's just an extra step where it's
easy to screw up your gel.  I know someone who has put a standard
acrylamide gel straight on the gel dryer and had no problems.  I've
always gotten nice clean bands with Long Ranger.

Jim



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