Pipette Tips, Summary
pfoster at bu.edu
Fri Jul 16 15:55:49 EST 1993
Sterilizing Pipette tips, Summary
Well this was fun. Everyone seemed to enjoy sharing his/her
inventions. I have two categories of information to share. First, dealing
with the non-autoclavable Combitips for Eppendorf repeater pipette.
The winning sterilization method came from Shoumo:
>I use these by sterilizing in 70% EtOH (just draw some up throw it out,
>and remove the EtOH by repeating the process with sterile water, LB, or
>whatever,) this works fine even for tissue culture.
Simple, elegant, cheap and effective (see below)
Lots of people just go ahead and autoclave them, although the
manufacturer warns that the plunger can crack. We found they survived
fine when the two parts are autoclaved together (15 min) but C Cho Yue
wraps them separately.
More information about the Methods