ECL and Western Blots
dotzlaw at ccu.umanitoba.ca
Tue Jul 20 09:56:18 EST 1993
In article <17906 at news.duke.edu>, lees at acpub.duke.edu (Steve Lee) wrote:
> I was wondering if anyone out there has used Amersham's ECL kit for
> western blot detection... is it as sensitive as they claim it is?
I have. I'm not sure if it's as sensitive as they "claim", but I can say
that it is definitely much more sensitive than AP. From zero signal on AP
to a "blasting" exposure using the ECL. It's less work than AP, too. We
use it all the time now.
> also, what's the maximun amount of protein that someone has successfully
> loaded onto Bio-Rad's Mini-Protean II?
I've gone up to 500 ug on a 1.5mm gel using the 10-well spacers.
More information about the Methods