(Forwarded) Re: sizing ssDNA

Ed Rybicki ED at micro.uct.ac.za
Wed Jul 21 04:45:23 EST 1993

Forwarded message:
From:     Self <MICRO/ED>
To:       mkennedy at chmeds.ac.nz (Martin Kennedy)
Subject:  Re: sizing ssDNA
Reply-to: ed at micro.uct.ac.za
Date:     21 Jul 93 12:06:50

> In article <CAFL9A.GHK at news.Hawaii.Edu>, marilou at ahi.pbrc.hawaii.edu (Marilou
> Andres) writes:
> > I have prepared ssDNA from pBS KS+ plasmid containing the f1 replication
> >site by infecting with the R408 phage. One of the ways some people suggested
> >that I canbe sure of what I have is indeed ssDNA is to run my sample along
> >with single stranded molecular weight marker in the gel.  However, such a
> >product does not seem to exist in the market.  Has anyone out there come
> >across a single stranded molecular weight market?  If not, is there any other
> >way of determining whether a DNA sample is single stranded?

S1 nuclease sensitivity....also, running it on a native and a denaturing
gel relative to dsDNA marker fragments.  Simple!  Same way we prove dsRNA
is and not ss.

 | Ed Rybicki, PhD             |       "Lord, won't you buy me        |
 | (ed at micro.uct.ac.za)        |                                      |
 | Dept Microbiology           |         A Mer-ce-des Benz..."        |
 | University of Cape Town     |                                      |
 | Private Bag, Rondebosch     |                                      |
 | 7700, South Africa          |           - Janis Joplin             |
 | fax: 27-21-650 4023         |                                      |

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