sizing ssDNA

Martin Kennedy mkennedy at chmeds.ac.nz
Wed Jul 21 00:58:18 EST 1993


In article <CAFL9A.GHK at news.Hawaii.Edu>, marilou at ahi.pbrc.hawaii.edu (Marilou 
Andres) writes:

> I have prepared ssDNA from pBS KS+ plasmid containing the f1 replication 
>site by infecting with the R408 phage. One of the ways some people suggested 
>that I canbe sure of what I have is indeed ssDNA is to run my sample along 
>with single stranded molecular weight marker in the gel.  However, such a 
>product does not seem to exist in the market.  Has anyone out there come 
>across a single stranded molecular weight market?  If not, is there any other 
>way of determining whether a DNA sample is single stranded? 
                                  
Why not just try sequencing some?  Running it on a gel isn't going to tell you
if its ss or ds, no matter what you use as markers.  You could maybe try
cutting it with a restriction enzyme too, as ss won't generally cut, but
contaminating rf (ds DNA) will. 

-- 
Cheers,

Martin

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