Competant Cells

Michael G. Tencza tencza at med.unc.edu
Fri Jul 23 07:57:19 EST 1993


I am preparing competant cells for the first time and have chosen the
"one-step" approach referenced above because it appears to be the most
convenient. I will be using E. coli strain HB101 and vector pBR322
(containing a 6kB insert). I would appreciate any hints from those who use
this method.



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