Plasmid size vs supercoiling

Martin Kennedy mkennedy at chmeds.ac.nz
Thu Jul 22 22:07:43 EST 1993


In article <1993Jul23.102604.282 at chmeds.ac.nz>, mkennedy at chmeds.ac.nz (Martin Kennedy) writes:
> In article <44814.jdboer at sol.uvic.ca>, jdboer at SOL.UVIC.CA ("Johan de Boer") writes:

> Plasmids often form dimers, which can't be resolved unless a specific
> site-specific recombination mechanism exists to resolve the dimer. 
 
I forgot to say this only happens in rec- hosts; if you use a rec+ host you
probably won't see the problem
                              
Cheers,

Martin

NNNN   NN  Martin A Kennedy (E-mail = mkennedy at chmeds.ac.nz)  ZZZZZZZ  
NN NN  NN       Cytogenetic and Molecular Oncology Unit          ZZZ
NN  NN NN           Christchurch School of Medicine            ZZZ
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