Screening Method for Large CCC Plasmids

[Russ Herwig]

Does anyone have a reliable method for the isolation and electrophoretic
separation of large (50-300 kb) ccc plasmids from bacteria?  We would like
to screen a culture collection consisting of environmental strains of
Vibrio and Pseudomonas spp. for large plasmids.

Russ Herwig
Department of Microbiology SC-42
University of Washington
Seattle, WA  98195