Dear Colleagues, I have 40 nmol of a 58 nucleotide oligo. I also have 40 nmol of an 18 nucleotide primer complementary to the 3' end of the oligo. I wish to double strand the oligo, but have not been able to find a good protocol. Is there a good one? How much can I do in one reaction? -Thanks -Len Adleman -USC -adleman at pollux.usc.edu