WANTED: Protocol to double strand oligo

Len Adleman adleman at pollux.usc.edu
Sun Jul 25 00:42:40 EST 1993


Dear Colleagues,

I have 40 nmol of a 58 nucleotide oligo. I also have 40 nmol of an 18 
nucleotide primer complementary to the 3' end of the oligo. I wish to
double strand the oligo, but have not been able to find a good protocol.
Is there a good one? How much can I do in one reaction?

-Thanks
-Len Adleman
-USC
-adleman at pollux.usc.edu



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