2x vector plasmid
JANIEMI at FINABO.ABO.FI
Mon Jul 26 00:52:36 EST 1993
In <2C5041E9 at adminbldg.lan1.umanitoba.ca> GIETZ at bldghsc.lan1.umanitoba.ca writes:
> I know for a fact that I have run across (years ago) a copy of pBR322
> that is a dimer!
> a number of plasmids with large direct repeats that seem stable in coli. I
> recover inverted repeats of any type! This is my $0.02 worth.
> Dan Gietz
Long inverted repeats are not stable in E. coli. This has been used to
construct positive selection vectors, see for instance Kieser & Melton,
Gene 65 (1988) 83-91. A whole plasmid ligated "head to head" probably
qualifies for a very long repeat...
Before I knew about this, I often wondered about some products, that one
surely must produce in ligations, but never sees.
Jarmo Niemi Biochemistry, University of Turku, Finland, janiemi at finabo.abo.fi
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