coady at ERE.UMontreal.CA
Mon Jul 26 19:16:19 EST 1993
In article <CAsD5r.A79 at ccu.umanitoba.ca> hamel at ccu.umanitoba.ca (Andre Hamel) writes:
>Have you tried out the method described by Nishimura et.al. (Nucl. Acids
>Res., Vol. 18, No.20, pg 6169, 1990) ... it's a GREAT method, yielding
>high efficiency competent cells ... follow their method to the letter ...
>is now a standard protocol in our lab.
Another method that I've used regularly (and swear by) is found
in Inoue et al, Gene vol. 96, pp. 23-28 (1990). It works very well and is
quite simple; the only drawback is growing bacteria at 18 C. I have
to lug my big water bath (including a pump) to the incubator and put a
rubber hose around my flask with a few coils, to keep the temperature
at 18. But the method is quite simple and works well.
Michael J. Coady
COADY at ERE.UMONTREAL.CA
"I don't got to show you no stinking .signature..."
More information about the Methods