Fill-in enzymes

greg at MENDEL.LLNL.GOV greg at MENDEL.LLNL.GOV
Mon Jul 26 13:04:49 EST 1993


We are searching for an enzyme that can reliably fill-in
5' overhangs such that they become blunt ends ... but
without ever losing even a base off the end.  Our luck so
far, even with 3'-5'exo lacking enzymes, has not been
great.  Would anyone have recommendations concerning either
the enzyme of choice and/or optimal reaction conditions ?

Many thanks,

Greg Lennon

****************************************************************

 
Greg Lennon
Human Genome Center, L-452
Lawrence Livermore National Lab
Livermore, CA 94550   USA
Email: greg at mendel.llnl.gov
Phone: (510) 422-5711  Fax: (510) 423-3608



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