ss oligos --> dsDNA

brett at BORCIM.WUSTL.EDU brett at BORCIM.WUSTL.EDU
Mon Jul 26 11:40:34 EST 1993


My protocol is to combine the oligos in a medium salt buffer (as 1x lign buffer)
containing Mg++. Heat to 92C for 2 min and slowly cool to anneal. Can then be
used directly in a ligation, etc. Also, you may as well try to match the
molarities.

Brett Lindenbach
Washington University - St. Louis
brett at borcim.wustl.edu



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