geneclean hassles

Michael G. Kurilla mgk2r at Virginia.EDU
Tue Jul 27 13:05:11 EST 1993


One problem you may be having with geneclean is due to the size
of your fragment (<1kb).  They are finicky.  For those, drop
the pH of the TAE running buffer and gel to 6.0 by adding more
GAA when you make it up.  I got this tip directly from Bio101
people.  I don't know if it's in the instructions, but for
small stuff it makes a difference.



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