Plant RNA extraction

braam at DINGO.RICE.EDU braam at DINGO.RICE.EDU
Tue Jul 27 09:16:52 EST 1993


>I will be working for the first time with plant tissue and cultured cells.  I 
>need to extract RNA from them, and the method I have been given involves a
>CsCl gradient spin.  Could the acid guanidinium phenol chloroform method of
>Chonzynski and Sacchi be used here?  Are there any plant-specific modifications
>needed?   Thanks
>Daniel Kim


Use the procedure described in Verwoerd, et al. (1989) Nucleic Acids
Research 17: 2362.  It's unbelievably easy and fast, and works extremely
well. 
Forget gradients.

Janet Braam
Rice University
braam at bioc.rice.edu





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