Chemiluminescent detection of Western Blots: Preferred Method?

David Martin x3175 dmartin at crc.ac.uk
Tue Jul 27 10:22:15 EST 1993


We have had excellent results using Amershams ECL with a variety of western
blot types (Ab-HRP, Ab-Biotin:avidin-HRP, ligand-biotin:avidin-hrp)

Just wash in TBS or similar (ie no gelatin) prior to adding the ECL reagents
after your usual probing method and you should get good results.

best of luck

....d
 



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