Chemiluminescent detection of Western Blots: Preferred Method?

William R. Morgan wmorgan at ACS.WOOSTER.EDU
Tue Jul 27 09:41:08 EST 1993


Dear Netters:

Any recommendations on the preferred method for nonisotopic detection with
Western blots?  

I plan to perform a Western blot using a recombinant anti-phosphotyrosine
antibody (Transduction Lab's RC20).  The RC20 Ab comes conjugated to HRP or
Alkaline Phosphatase.   Transduction Lab provides a chemiluminescent
detection protocol for their HRP-conjugated Ab using ECL (but only a
NBT/BCIP detection protocol for their AP-conjugated Ab).  Is Amersham's
Western Blotting Detection System recommended or is an an alkaline
phosphatase system (e.g. USB's Protein Images with LumiPhos reagent) 
preferred?  Thanks for any help.

Bill Morgan

William R. Morgan
The College of Wooster
Department of Biology
931 College St.
Wooster, OH  44691
Phone:  216-263-2026
FAX:    216-263-2378
E-mail: wmorgan at acs.wooster.edu




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