Competant Cells

dkim at dkim at
Wed Jul 28 09:42:07 EST 1993

For a really quick and dirty transformation (e.g. when you receive plasmid 
from someone and want to grow it up), there was a BioTechniques method in
which plasmid is simply added to fresh log phase bugs and the whole mess
is frozen for 1 minute in liquid nitrogen.  Thaw at room temperature and spread 
the bugs on a plate.  That's all.  Daniel Kim

More information about the Methods mailing list