Competant Cells

dkim at triton.unm.edu dkim at triton.unm.edu
Wed Jul 28 09:42:07 EST 1993


For a really quick and dirty transformation (e.g. when you receive plasmid 
from someone and want to grow it up), there was a BioTechniques method in
which plasmid is simply added to fresh log phase bugs and the whole mess
is frozen for 1 minute in liquid nitrogen.  Thaw at room temperature and spread 
the bugs on a plate.  That's all.  Daniel Kim



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