PCR of CMV in Tissue Culture

Chris Upton cup at bones.biochem.ualberta.ca
Tue Jul 27 22:20:49 EST 1993


In article <btaylor.743798979 at muskwa.ucs.ualberta.ca>,
btaylor at muskwa.ucs.ualberta.ca (Taylor Brian) wrote:
> 
> Hi.  I'd like to PCR a piece of CMV DNA from CMV-infected tissue
> culture cells. I've already made my primers, now I'm interested
> in finding the best way to harvest the virus, get the DNA, etc.
> Can anyone help me?
> 
> Thanks in advance
> 
> Brian Taylor
> Dept. of Medical Microbiology and Infectious Diseases
> University of Alberta,  CANADA
> 
> i
HI Brian,
         Have you tried freeze-thaw? I've done this with vaccinia infected
cells. Scrape into buffer, freeze-thaw a couple of times, spin out the junk
and try PCR with a couple of ul.
Chris Upton
University of Alberta

As of Sept 1st 1993,  cupton at sol.uvic.ca
Department of Biochemistry & Microbiology
University of Victoria         
PO BOX 3055, Victoria          
BC V8W 3P6                     
Canada                 
(604)721-6507 phone        
(604)721-8855 fax



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