PCR of CMV in Tissue Culture
cup at bones.biochem.ualberta.ca
Tue Jul 27 22:20:49 EST 1993
In article <btaylor.743798979 at muskwa.ucs.ualberta.ca>,
btaylor at muskwa.ucs.ualberta.ca (Taylor Brian) wrote:
> Hi. I'd like to PCR a piece of CMV DNA from CMV-infected tissue
> culture cells. I've already made my primers, now I'm interested
> in finding the best way to harvest the virus, get the DNA, etc.
> Can anyone help me?
> Thanks in advance
> Brian Taylor
> Dept. of Medical Microbiology and Infectious Diseases
> University of Alberta, CANADA
Have you tried freeze-thaw? I've done this with vaccinia infected
cells. Scrape into buffer, freeze-thaw a couple of times, spin out the junk
and try PCR with a couple of ul.
University of Alberta
As of Sept 1st 1993, cupton at sol.uvic.ca
Department of Biochemistry & Microbiology
University of Victoria
PO BOX 3055, Victoria
BC V8W 3P6
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