David Martin x3175
dmartin at crc.ac.uk
Wed Jul 28 11:43:09 EST 1993
We are considering making selenomethionine proteins using met- E.Coli.
Does anyone have any tips for purification? (we have a good method for
purifying the S-met variant of our protein.)
I heard somewhere that it is important to have 10mM DTT in the purification
buffers but this is not desirable.
Does anyone have any experience with purifying selenomethionine proteins?
thanks in advance
Haemostasis research group
Clinical Research Centre
dmartin at uk.ac.crc
More information about the Methods