Competant Cells

Paul N Hengen pnh at fcsparc6.ncifcrf.gov
Wed Jul 28 13:23:28 EST 1993


In article <23637vINNl71 at lynx.unm.edu> dkim at triton.unm.edu () writes:

> For a really quick and dirty transformation (e.g. when you receive plasmid 
> from someone and want to grow it up), there was a BioTechniques method in
> which plasmid is simply added to fresh log phase bugs and the whole mess
> is frozen for 1 minute in liquid nitrogen.  Thaw at room temperature and spread 
> the bugs on a plate.  That's all.  Daniel Kim

I just quickly scanned BioTechNet. This may also be of some help:

1  (#3-0105)COMPARISON OF VARIOUS COMPETENT CELL PREPARATION METHODS ...

TITLE  : COMPARISON OF VARIOUS COMPETENT CELL PREPARATION METHODS
AUTHOR : LIU, HAIYING, RASHIDBAIGI, ABBAS
KEYWORD: COMPETENT CELLS, E. COLI, TRANSFORMATION

COMPARISON OF VARIOUS COMPETENT CELL PREPARATION METHODS FOR HIGH EFFICIENCY DNA
TRANSFORMATION.

(Short Technical Report, Biotechniques, Vol.8, No.1, p.21-25)

Paul N. Hengen
National Cancer Institute
Frederick Cancer Research and Development Center
Frederick, Maryland 21702-1201 USA



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