Competant Cells

[Klaus.Matthaei at anu.edu.au]

>For a really quick and dirty transformation (e.g. when you receive plasmid 
>from someone and want to grow it up), there was a BioTechniques method in
>which plasmid is simply added to fresh log phase bugs and the whole mess
>is frozen for 1 minute in liquid nitrogen.  Thaw at room temperature and
>spread 
>the bugs on a plate.  That's all.  Daniel Kim

Here is another dirty one if your plasmid is in the wrong bug on a plate
e.g. if you want ssDNA for site directed mutagenesis and the bug it's in
wont generate ssDNA.  (Biochimica Brief a couple of years back)  Take a
toothpick sample of the colony and smear into an eppendorf tube.  Boil 10
min, add new bugs, vortex and plate out.  Works in most cases.

Cheers, Klaus
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Klaus Matthaei
Gene Targeting
The John Curtin School of Medical Research
The Australian National University
E-mail: Klaus.Matthaei at anu.edu.au

"I know that you think that you think that you understand what I am saying."
"But what I am saying is sometimes not actually what I mean."
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