Formation of plasmid oligomers

Martin Kennedy mkennedy at
Wed Jul 28 22:06:59 EST 1993

For those of you still wondering about formation of plasmid dimers, here are
some early references:

Hobom and Hogness (1974)	 J.Mol.Biol. 88, 65-87
Bedbrook and Ausubel (1976)	 Cell 9, 707-716
Potter and Dressler (1977)  	 PNAS 74, 4168-4172

In a nutshell, these papers demonstrated that oligomeric molecules comprised of
tandem repeats of a monomer plasmid can arise by recA dependent recombination
events.  When the different forms were isolated and transformed back into recA-
strains, the multimers retained their initial conformation.  Monomeric forms
transformed into recA- strains don't generate oligomeric forms.  I guess what
I'm trying to say is that this phenomenon has been around so long just about
everyone has forgotten about it, hence the confusion when someone "rediscovers"
plasmid dimers!!



NNNN   NN  Martin A Kennedy (E-mail = mkennedy at  ZZZZZZZ  
NN NN  NN       Cytogenetic and Molecular Oncology Unit          ZZZ
NN  NN NN           Christchurch School of Medicine            ZZZ
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