PCR with degenerate primers

[Nelson Salazar]

Hello there,

I would like to know what parameters should I change in order to
improve the possibility of identifying a specific gene by using
degenerate primers ??

I have lowered the annealing temperature to give the primers more
chance to anneal but I would like to hear some advice from the
experts on PCR, and specifically those who have used it to identify
new genes from total DNA. Thanks very much in advance for your help.

                            N. Salazar






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Nelson Salazar                      *  E-mail: n.salazar at lshtm.ac.uk
Dept. of Medical Parasitology       *    or    nsalazar at mrc-crc.ac.uk
Lon. Sch. Hyg. & Trop. Med.         *  Phone : 071 927 2468 (Office)
Keppel St. London WC1E 7HT, UK      *  Fax   : 071 636 8739
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