G + C rich sequences

Baldauf at imb.lan.nrc.ca Baldauf at imb.lan.nrc.ca
Fri Jul 30 09:36:00 EST 1993


>formamide in 5xSSC + 5x Denhardts + 1% SDS + 10% dextran sulphate +
>250micrograms/ml of salmon sperm DNA at 42 degrees; we wash with 0.1X SSC +

You might want to try decreasing the dextran sulphate, it's notorious for
giving background.  Use 5% or even leave it out altogether.  Unless you're
looking for something very rare in a large complex genome, or using a poorly
matched probe, you probably don't need it.



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