Problems with Westerns

Ed Rybicki ED at micro.uct.ac.za
Fri Jul 30 03:02:43 EST 1993


>      On a related front, we have tried stripping antibodies
> off nitrocellulose blots with elevated temperatures and SDS
> following published protocols and then reprobing with
> different antibodies.  It works fine, but when we try this
> with PVDF membranes the blotted proteins come off too.  Has
> anyone a method for stripping of such blots and reuse?

Try soaking blot in 0.1M glycine/HCl pH 2.9, containing (or not) 0.15M
NaCl.  Ab should come off pretty well.

  ____________________________________________________________________
 | Ed Rybicki, PhD             |       "Lord, won't you buy me        |
 | (ed at micro.uct.ac.za)        |                                      |
 | Dept Microbiology           |         A Mer-ce-des Benz..."        |
 | University of Cape Town     |                                      |
 | Private Bag, Rondebosch     |                                      |
 | 7700, South Africa          |           - Janis Joplin             |
 | fax: 27-21-650 4023         |                                      |
  --------------------------------------------------------------------






More information about the Methods mailing list