transforming with RE digest

g_wenck at spike.psc.scarolina.edu g_wenck at spike.psc.scarolina.edu
Fri Jul 30 13:18:39 EST 1993


I've digested ligation mixtures after heat inactivation of the ligase
and have used the mixture for transformation with a lot of sucess.
this technique realy helps when your cloning step destroys a cutting
site and you do not have a convenient marker for selection (ie blue\
white).



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