Boiling Method for Minipreps

rjb at canctr.mc.duke.edu rjb at canctr.mc.duke.edu
Sat Jul 31 12:53:28 EST 1993


In article <23c14a$r60 at samba.oit.unc.edu>, tencza at med.unc.edu (Michael G. Tencza) writes:
>I would like to know if anyone uses the boiling method for plasmid
>minipreps to prepare DNA for sequencing.  I wish to try this method for
>several pBR322 constructs I have recently transformed into HB101.  Any
>hints would be appreciated.
No, I have never used a "boiling" miniprep for ds-sequencing. Instead, I
have got consistently excellent results using the method described by 
Johnson (Anal. Biochem. 190:170-174 (1990)) followed by PEG-precipitation.
Good luck.
Rohan de Silva.



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