phagemids vs plasmids
isbruck at ac.dal.ca
isbruck at ac.dal.ca
Wed Jun 2 12:37:16 EST 1993
Hello netters:
I recently received a cDNA clone that is inserted in a phagemid
vector. Can the phagemid be grown, amplified and isolated using
the same methods used for plasmid isolation? I am currently
isolating my plasmids using a CsCl/ultracentrifuging method but
am not too sure if this will work for a phagemid. Also, are
there any good references on phagemids? the only thing I have
read is the short blurb in the Maniatis volumes, but this does
not have very much on the methods.
Thanks very much,
Richard
--
Richard Isbrucker e-mail: ISBRUCK at AC.DAL.CA
Dalhousie University, Tel: (902) 494-2571
Dept of Pharmacology
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