jishikaw at ddbj.nig.ac.jp
Thu Jun 3 13:48:20 EST 1993
I am trying to sequence Streptomyces DNA (GC%=>70%) with USB cycle
sequencing kit. But I can not obtain only very weak signals.
My protocol is as follows:
Labeling reaction: 95c, 15sec
x 30 cycles
Termination reaction: 95c, 30sec
x 30 cycles.
Double-stranded DNA (upto 2-kb) inserted in pUC plasmid were subjected
Does anyone have any idea or suggestion about the cycle sequencing of
high GC content DNA?
Thank you in advance.
Jun Ishikawa, Ph.D. (Mr.) Dept. of Bioactive Molecules
NATIONAL INSTITUTE of HEALTH
jishikaw at ddbj.nig.ac.jp 1-23-1, Toyama, Shinjuku,
Tokyo 162, Japan
P.S. Sorry for my poor English.
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