help: neomycin-resistance gene

Jonathan W. Jarvik jj03+ at andrew.cmu.edu
Thu Jun 3 12:26:15 EST 1993


We want to construct a vector that will confer kanamycin/neomycin
resistance in Chlamydomonas by expressing the neomycin
phosphotransferase gene. The vector we've designed has a Chlamydomonas
promoter and polyadenylation signal.  What we need is the npt coding
sequence complete with a translation start that will work in a
eucaryote. Can anyone help? 

Please reply to jj03+ at andrew.cmu.edu

Thanks, Jon Jarvik, Dept. Biological Sciences, Carnegie Mellon University.



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