Problems with Promega Taq

[Andre Hamel]

(stuff deleted from original posting)

>> Have you tried Deep Vent exo- from New England Biolabs?
                            ^^^^


>I've tried side by side tests with NEB's Vent (neither Deep nor
>Exo-) and it seems that for my setup, Taq makes a lot more
>product than Vent.

But this comparison would be "more valid" if Taq were compared with the
exo- versions of the Vent "family" (V and DV) ... Vent gives poor yields,
DV even worse, whereas Vexo- and DVexo- give at least as good as Taq (in
most cases better, although I never "properly" calibrated, just "eye ball"
based on band intensities ... Taq requires MORE enzyme (usually at least a
unit per 100 uL rx'n), whereas Vexo- and DVexo- work great at lower
enzyme conc. (0.2 to 0.5 units/100 uL rx'n) ... factor this with the unit
cost ... one is less apt to feel like a "hostage" of a company that
appears unwilling to further broaden their product lines for the
betterment of researchers, not JUST corporate "bottom line" ... 

regards, Andre
                            ********************
Andre Hamel                                 email: hamel at ccu.umanitoba.ca
Manitoba Veterinary Services                lab tel.: (204) 945-7630
Infectious & Genetic Disease                     FAX: (204) 945-8062     
Mol.Biol.Lab., Winnipeg, Manitoba, CANADA
"I'm not politically incorrect, just euphemistically challenged"
                            ********************