sequencing at high temp
thomas at molbio.uoregon.edu
Thu Jun 3 19:17:57 EST 1993
I have a stech of DNA I have been trying to sequence but I am encountering
difficulties. I think this is due to secondary structure present in the
single strand DNA. I have tried sequenceing with ITP but with no luck.
anyone out there have a protocol for sequencing with Taq polymerase or
someother high temp stable enzyme. I think If I did the reactions at a
high temp this might solve my problem.
Thanks in advance,
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