sequencing at high temp

Matthew Thomas thomas at
Thu Jun 3 19:17:57 EST 1993

I have a stech of DNA I have been trying to sequence but I am encountering
difficulties.  I think this is due to secondary structure present in the
single strand DNA.  I have tried sequenceing with ITP but with no luck. 
anyone out there have a protocol for sequencing with Taq polymerase or
someother high temp stable enzyme.  I think If I did the reactions at a
high temp this might solve my problem.

			Thanks in advance,
										Matt Thomas


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