sequencing at high temp

Matthew Thomas thomas at molbio.uoregon.edu
Thu Jun 3 19:17:57 EST 1993


I have a stech of DNA I have been trying to sequence but I am encountering
difficulties.  I think this is due to secondary structure present in the
single strand DNA.  I have tried sequenceing with ITP but with no luck. 
Does
anyone out there have a protocol for sequencing with Taq polymerase or
someother high temp stable enzyme.  I think If I did the reactions at a
high temp this might solve my problem.

			Thanks in advance,
										Matt Thomas


     



More information about the Methods mailing list