Vent et.al polymerase for PCR

[Patricia Foster]

Zoltan Penzes (PENZES at IRAD.AFRC.AC.UK) wrote:
: Reading so many good things about the Vent polymerase family,
: I am wondering whether Vent (or Vexo, DVexo) actually
: nibbles the ends of the PCR product.
  
  I plan to summarize what I have learned about Promega Taq soon,
but I do like Vent.  Exo+ will nibble ends, but this can be solved
by keeping the dNTPs high (400uM).  No problem so far with exo-


: I there a "learning curve" using Vent, as we've found with Pfu?

  Yep.  Playing around with the Mg concentrations helps.
--
Patricia L. Foster
Boston University School of Medicine
Boston, MA USA
pfoster at bu.edu