Vent et.al polymerase for PCR
pfoster at bu.bu.edu
Fri Jun 4 18:53:45 EST 1993
Zoltan Penzes (PENZES at IRAD.AFRC.AC.UK) wrote:
: Reading so many good things about the Vent polymerase family,
: I am wondering whether Vent (or Vexo, DVexo) actually
: nibbles the ends of the PCR product.
I plan to summarize what I have learned about Promega Taq soon,
but I do like Vent. Exo+ will nibble ends, but this can be solved
by keeping the dNTPs high (400uM). No problem so far with exo-
: I there a "learning curve" using Vent, as we've found with Pfu?
Yep. Playing around with the Mg concentrations helps.
Patricia L. Foster
Boston University School of Medicine
Boston, MA USA
pfoster at bu.edu
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