Problems with Promega Taq
pfoster at bu.bu.edu
Sat Jun 5 18:48:54 EST 1993
: Promega's Taq doesn't like Cetus buffer, and the presence of detergent in
: the Promega buffer may be the reason, altho I haven't tried to add
: detergent to the Cetus stuff.
Jack Kramer wrote:
:I had similar problems with the Promega Taq with <Promega's 10X
:buffer. The Cetus Taq had similar problems in the same buffer.
:Switching to the Cetus buffer cured the problem. With this buffer,
:or modifications of it which <i make up myself now, I get identical
:results with either Taq. Try Promega Taq in storage buffer B and make
:up your own reaction buffers - saves bundles of money!
Huh? Could we have the recipes?
Patricia L. Foster
Boston University School of Medicine
Boston, MA USA
pfoster at bu.edu
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