cloning and purity of DNA
genpt at strix.udac.uu.se
Mon Jun 7 06:47:17 EST 1993
After trying DNA fingerprinting on my Bumblebees without any variation
at all (with 4 probes) I'm now planning to develop microsatellite markers
instead. Since I have never cloned DNA I have some questions:
1. How clean does my bumblebee DNA need to bee? In prior experiments I have
used Phenol/Chloroform extraction (one extraction with warm Phenol) and
then I have reprecipitated my DNA once. Is this enough?
2. I am planning to use Boehringers pUC Cloning kit. Comments?
3. Do someone out there have any tips and hints that may be of use to me?
Thanks in advance.
Box 7003; S-750 07 Uppsala; Sweden
Phone: +46-18 67 13 23
FAX: +46-18 67 27 05
E-mail: Peter.Thoren at genetik.uu.se
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