Storage of RNA

Ola Myklebost olam at radium.uio.no
Tue Jun 8 04:13:37 EST 1993


In article <1uvjo0INN1l5 at post.its.mcw.edu>, dmeier at post.its.mcw.edu (Daniel
Meier) wrote:
> 
> Peter Kristensen (ptk at biobase.aau.dk) wrote:
> : Hi Netters.
> : Any comments and suggestions on how to store my RNA so that I can keep a 
> : reliable pool of standard RNA to add.
> 
> Peter,
> 
>   Store your RNA in the appropriate concentrations in DEPC-treated water
> at - 70 C.  Aliquot the RNA so that you thaw each tube once and discard. 
> I wouldn't refreeze the RNA.
> 
There shouldn't be any problems freezing and thawing the RNA several
times, but aliquoting is of course a good idae.

Ola Myklebost             Email olam at radium.uio.no
Dept of Tumor Biology
Inst for Cancer Research
The Norwegian Radium Hospital
N-0310 OSLO, Norway
Tel +47-22-50-60-50, xtn 9830, Fax +47-22-52-24-21



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