rna at -40 to -80 ?

Martin Kennedy mkennedy at chmeds.ac.nz
Tue Jun 8 21:55:35 EST 1993


In article <1993Jun8.185521.3689 at gserv1.dl.ac.uk>, suter at VAX.MPIZ-KOELN.mpg.d400.de writes:
> 
> why is it that everybody wants to store there rna at -80 ? in my opinion,
> diffusion is reduced to zero at -20, and therefore rnases won't be very active at
> that temperature. 
> i store all nucleic acids at -20: i have never noticed any drop in quality.
> repeated freezing and thawing will damage nucleic acids much more; that
> is because of the presence of nucleases, but also because of the physical
> forces and chemically instability during these two processes.
> 
> 
> clemens
-- 
I agree completely!!  In practice though, I don't bother aliquotting the RNA
and I've never noticed any appreciable damage after several freeze-thaws.

Cheers,

Martin

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