PFGE agarose plugs
nash at biologysx.lan.nrc.ca
Wed Jun 9 10:04:12 EST 1993
What do people use for agarose plug formers when they are embedding cells in
agarose prior to lysis, restriction digests, etc for PFGE? Do I have to
go out and buy an expensive mould? I got tech. support to make a rather
nice mould, such that the plugs will fit in my gel wells, but the plugs are
1 mm thin and rather brittle. Should I perservere, or is there a nice trick
with a syringe, or something?
If you email replies to me, I'll summarize and post.
John Nash | Email: Nash at biologysx.lan.nrc.ca.
Institute for Biological Sciences |
National Research Council of Canada | Email to my other NRC accounts
Ottawa, Ontario, Canada. | is usually forwarded here.
*** Disclaimer: All opinions are mine, not NRC's! ***
More information about the Methods