autorad cassettes (fwd)
rapr at MED.PITT.EDU
Wed Jun 9 21:34:32 EST 1993
> In article <1v0tvt$2fc at uniwa.uwa.edu.au> mitch at uniwa.uwa.edu.au
> (Michelle Waycott) writes:
> >What is the situation regarding autoradiograph cassettes. I have only
> >used the (very) expensive light sealed cassettes such as that from Fuji
> >but I believe that there are alternatives were you might buy the
> >intensifier screens and a carboard "sleeve" which is light sealed.
> >These can then be exposed at -80C.
> >Is this procedure used regularly by some groups and if so any
> >suggestions about suppliers and or sources of screens and "sleeves"
> >would be helpful.
> All I know about this I learned from Ron Laskey's articles, e.g.
> Meth Enzymol 65:363-371, 1980.
> The cardboard cassettes are available from Sigma (among other suppliers)
> but really need to be clamped between two boards to be suitable for -80 C
> storage. Compared to the cost of the intensifying screems <- Freudian
> slip, but apt :-) screens, the cassettes don't seem so bad.
> Hope this helps
> Jim Hutchins  E-Mail: jbh at anat.umsmed.edu
> Asst Prof of Anatomy  Asst Prof of Neurology
> Univ Mississippi Med Ctr  Jackson, MS
We agree: our cardboard cassettes are kodak products; we sandwich them
between same-size sheets of 1/4 inch plywood using giant binder-clips,
one on each edge, for -80C with cronex (sp?) intensifier screen.
Robert A. Preston
UPMC Dept. Pathology
rapr at med.pitt.edu
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