autorad cassettes (fwd)

Robert Preston rapr at MED.PITT.EDU
Wed Jun 9 21:34:32 EST 1993


> 
> In article <1v0tvt$2fc at uniwa.uwa.edu.au> mitch at uniwa.uwa.edu.au
> (Michelle Waycott) writes:
> 
> >What is the situation regarding autoradiograph cassettes.  I have only
> >used the (very) expensive light sealed cassettes such as that from Fuji
> >but I believe that there are alternatives were you might buy the
> >intensifier screens and a carboard "sleeve" which is light sealed. 
> >These can then be exposed at -80C.
> >Is this procedure used regularly by some groups and if so any
> >suggestions about suppliers and or sources of screens and "sleeves"
> >would be helpful.  
> >
> 
> All I know about this I learned from Ron Laskey's articles, e.g.
> Meth Enzymol 65:363-371, 1980.
> 
> The cardboard cassettes are available from Sigma (among other suppliers)
> but really need to be clamped between two boards to be suitable for -80 C
> storage.  Compared to the cost of the intensifying screems <- Freudian
> slip, but apt :-) screens, the cassettes don't seem so bad.
> 
> Hope this helps
> Jim
> 
> -- 
> Jim Hutchins                    []     E-Mail: jbh at anat.umsmed.edu
> Asst Prof of Anatomy            []     Asst Prof of Neurology
> Univ Mississippi Med Ctr        []     Jackson, MS
> 
We agree: our cardboard cassettes are kodak products; we sandwich them
between same-size sheets of 1/4 inch plywood using giant binder-clips,
one on each edge, for -80C with cronex (sp?) intensifier screen.

Robert A. Preston
UPMC Dept. Pathology
rapr at med.pitt.edu





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