differential screening/display

[Basavaraju Shankarappa]

Check the article by Liang and Pardee; Differential display of eukaryotic
mRNA by means of PCR.  Science 257:967.  I think it should give you
very good pointers as an alternative to substractive libraries
or other such things.
Raj Shankarappa
bsh at med.pitt.edu
Univ of Pittsburgh
> 
> Dear Netters,
> 
> I am interested in differential screening to isolate new genes that are
> expressed in one part of the embryo and not another. The problem is the
> extremely tiny pieces of tissue that one has for RNA isolation. The
> dissection is not a problem however. 
> 
> Has anyone out there used PCR successfuly to generate a cDNA library? Does
> Taq have problems amplifying 2.0 kb + fragments? Are there sequencing
> errors introduced?
> 
> Any relevant references and suggestions are welcome. 
> 
> I already have copies of these articles: 
> 
> Brunet et al, 1991, Science, vol.252, pg 856-859. 
> 
> Straus and Ausubel, PNAS, 87, 1889-1893. 
> 
> Belyavsky et al, NAR, vol.17, 1989, 2919-2931
>  
> 
> -- 
> The most important event in life is not birth or death but gastrulation.
> Lewis Wolpert
> jrichmn at ccu.umanitoba.ca
>