ALSOBROOK at biomed.med.yale.edu
Thu Jun 10 16:40:22 EST 1993
Well, I found these four refs in the "older" literature on Ethidium Bromide,
but unfortunately only one had an abstract available online. The abstract
is enough to convince me not to drink Ethidium Bromide, and to doubt any
stories about someone drinking a batch...
AU - Higgins ES, Dunlavey BL, Friend WH, Rogers KS
TI - Ethidium bromide inhibits mitochondrial phosphorylating oxidation.
SO - Proceedings of the Society For Experimental Biology & Medicine 1975
AB - Ethidium bromide, in addition to combination with mitochondrial
nucleic acids, is a phosphorylation inhibitor during glutamate and
succinate respiration by mitochondria. Exhaustive washing of
ethidium bromide-treated mitochondria did not relieve the inhibition
nor significantly decrease the amount of bound dye. Dialysis against
a cation exchange resin at 3 degrees for 17 hr removed about 97% of
bound dye. This restored phosphorylating capacity to that of
untreated mitochondria which had also been dialyzed against the
resin. Since state 3 respiration was diminished and state 4 was
unaffected by the presence of the acridine dye, and since neither
swelling of mitochondria nor release of latent ATPase was observed,
then ethidium bromide was not an electron transport inhibitor nor an
uncoupler of oxidative phosphorylation. Inhibition of metabolic
processes by ethidium bromide may be due in part to depressed
generation of mitochondrial ATP.
AU - King ME, Godman GC, King DW
TI - Respiratory enzymes and mitochondrial morphology of HeLa and L cells
treated with chloramphenicol and ethidium bromide.
SO - Journal of Cell Biology 1972 Apr;53(1):127-42
AU - Khan A, Hill JM
TI - Immunosuppression with ethidium bromide: effect on lymphocyte
blastogenesis and antibody plaque forming spleen cells.
SO - Experimental Hematology 1973;1(2):119-22
AU - Darke C, Gay E
TI - An evaluation of the ethidium bromide microcytotoxicity test.
SO - Journal of Immunological Methods 1974 Mar;4(2):161-72
* John Alsobrook Child Study Center Yale University School of Medicine *
* internet: alsobrook at biomed.med.yale.edu / bitnet: alsobrook at yalemed.bitnet *
* "Giving money and power to the government is like giving whiskey and car *
* keys to teenage boys." -- P.J. O'Rourke *
More information about the Methods