Problems with Promega Taq

Vivian Miao VMiao at oregon.uoregon.edu
Thu Jun 10 17:26:22 EST 1993


In article <1v57hjINNio6 at lynx.unm.edu>, bhjelle at carina.unm.edu () wrote:
> In article <Miao-030693075250 at fp1-molbio-13.uoregon.edu> Miao at oregon.uoregon.edu (Vivian Miao) writes:

>> side by side tests with NEB's Vent...  for my setup,
>>Taq makes a lot more product than Vent

>>the reactions are made up as a cocktail minus
                                                ^^^^^^^^^^^^^^
>>enzyme and its conc'd buffer stock which are added later, the
^^^^^^^

> We normally make sure there are some salts in the
> reaction mix before we add the enzyme, on the assumption
> that diluted enzyme may denature in a situation where
> there is no salt or buffer. Maybe all you are seeing is
> a differential sensitivity to denaturation of Vent compared
> with Taq.
> 
> Brian

sorry, bad editing on my part in the original post  
First cocktail = template, oligos, NTP's, water.
Then salts, 
Then enzyme
(Then oil)

(Then take bets on what will go wrong this time, etc. ...:-)

Vivian



More information about the Methods mailing list