Native PAGE

[Ding Ming]

>I am trying to detect a protein complex using native gel
>electrophoresis.  The isoelectric point of of one of the proteins
>is about eight,  the others have not been identified.  After

Obviousely, your sample is alkalinic or near basic. You should use a 
acidic buffering system to run. I remember there is a book 
called"Disc Electrophoresis and related techniques of PAGE" written 
by H. R. Maurer in 1971, which described a very good acidic 
buffering system for native PAGE of basic proteins at pH 4.5. Hope 
you can find it.

Good luck!



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    Ding Ming                     
    (608)265-3544                   ming at calshp.cals.wisc.edu
    Sweet Protein Section           ming at zeus.ahabs.wisc.edu
    Taste Research Lab               
    Department of Animal Health & Biomedical Sciences    
    University of Wisconsin-Madison, 1655 Linden Dr., Madison, WI53706      
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