Joyce-Leung Random PCR Mutagenesis

the End jgraham at bronze.ucs.indiana.edu
Wed Jun 16 08:31:02 EST 1993


Techniques which take advantage of the lack of proof reading in the 
Taq polymerase seem to be catching on. I am interesting in how the 
randomization mutagenesis procedure described by Leung is working 
for people out in the field. Note this is not a chemical or site
directed approach, but simply a PCR reaction with error-prone conditions.

If you have used this technique, please drop me a note.

Thanks much,

Jim
J. Graham
Richardson Biochemistry Laboratory



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