Ethidium Bromide

JIM MANHART manhart at
Wed Jun 16 17:38:00 EST 1993

In article <C8MK2x.B70 at>, browns at
(Stuart Brown) writes...
>I once had a protocol for the "safe destruction" of EtBr.  It invlved
>acidification and then neutralization with baking soda.  Anyone out there
>know this one (I'd like to get the ref. as we also have lots of jugs of
>EtBr waiting for me to get around to getting rid of it.) 

Not sure of the title, but:

Adams, Elhardt, and Scouten.  1988.  Phytochem. Bull. 20(2):18-19

also see Maniatis, 2nd ed. pp E.8-E.9

The procedure uses hypophosphorous acid (expensive, but a bottle lasts us for
a while), sodium nitrite, and sodium bicarbonate.

David Northcliffe, Research Assistant		(email: dln4300 at
Lab of James Manhart
Dept. of Biology - TAMU

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