Ethidium Bromide

[JIM MANHART]

In article <C8MK2x.B70 at ccu.umanitoba.ca>, browns at ccu.umanitoba.ca
(Stuart Brown) writes...
>I once had a protocol for the "safe destruction" of EtBr.  It invlved
>acidification and then neutralization with baking soda.  Anyone out there
>know this one (I'd like to get the ref. as we also have lots of jugs of
>EtBr waiting for me to get around to getting rid of it.) 
> 

Not sure of the title, but:

Adams, Elhardt, and Scouten.  1988.  Phytochem. Bull. 20(2):18-19

also see Maniatis, 2nd ed. pp E.8-E.9

The procedure uses hypophosphorous acid (expensive, but a bottle lasts us for
a while), sodium nitrite, and sodium bicarbonate.



------------------------------------------------------
David Northcliffe, Research Assistant		(email: dln4300 at venus.tamu.edu)
Lab of James Manhart
Dept. of Biology - TAMU