RT-PCR: which enzyme(s) are best?

David J. Meyer ez005587 at chip.ucdavis.edu
Thu Jun 17 17:54:13 EST 1993


Hello, netters!

	I need to do some RT-PCR soon, and am planning things now. I have
previously used MMLV RTase, followed by Taq for the PCR; does anyone have
any experience/recommendations regarding the use of enzymes such as Cetus'
rTth polymerase? It would be nice, in theory, to use just the one enzyme.
Please post replies, as I read this group regularly.

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David J. Meyer
djmeyer at ucdavis.edu



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