Speed-Vac rendundancy

John Jones john at tbone.biol.scarolina.edu
Thu Jun 17 20:18:09 EST 1993


In article <C8sCpn.K8J at usenet.ucs.indiana.edu> jgraham at bronze.ucs.indiana.edu (the End) writes:
>I am always finding that y'all go to the trouble to dry your nucleic acid
>precipitates "down" with a "Speed-Vac". Next time rinse your pellet with
>70% ethanol and set it upside down in a 50-60 C oven for five minutes. 
>You don't need a cannon to swat a fly. We don't even have a "Speed-Vac"
>and have been doing nucleic acid work without one for twenty years. Besides
>overdrying samples by this method leads to reduced recovery, and in fact 
>denatures short DNA fragments.


I agree. We find it even easier just to sit the tube under a bench lamp with 
its lid open for 5 minutes - gets most of the ethanol off as long as you 
remove the majority with a pipette first. 

 John

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>Jim
>J. Graham
>Richardson Biochemistry Laboratory
>Indiana University
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