Speed-Vac rendundancy

the End jgraham at bronze.ucs.indiana.edu
Thu Jun 17 16:51:22 EST 1993


I am always finding that y'all go to the trouble to dry your nucleic acid
precipitates "down" with a "Speed-Vac". Next time rinse your pellet with
70% ethanol and set it upside down in a 50-60 C oven for five minutes. 
You don't need a cannon to swat a fly. We don't even have a "Speed-Vac"
and have been doing nucleic acid work without one for twenty years. Besides
overdrying samples by this method leads to reduced recovery, and in fact 
denatures short DNA fragments.



Jim
J. Graham
Richardson Biochemistry Laboratory
Indiana University
.



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